Closed / Perfusion Cell Culture System
Closed and Perfusion Culture
Closed Culture
This is the culture method without opening or closing the completely sealed container, reservoir and tubing. This method can maintain the contamination-free and not affect gas condition from outside of the system.
This advantage makes easy to control the environment of medium by using an automated system.
Perfusion Culture
This is the automated cell culture method that generates continuously flow fresh culture medium into the container and remove old medium.
This method can maintain the condition of medium
(e.g. pH, concentration of the substance) and mimic in vitro condition and also can reduce labor and costs.
Closed × Perfusion
The combination of the two culture methods can solve many problems of automated cell culture.
This technique has the potential of reducing the cost of manufacturing the cells,
because of eliminating clean rooms and reducing human labor.
It also eliminates variations caused by manual work, leading to more consistent quality.
iP-TEC is actively advancing the development of “closed system perfusion culture system
” leveraging our container and perfusion technology expertise.
We automatically established hiPSC from only 1mL whole blood (WB) using our closed culture devices.
After separating PMBC from WB using density gradient centrifugation, we used Sendai virus vector for gene transfer. (Oct3/4, Sox2, Klf4, c-Myc)
Finally,
iPS cell-specific colonies and the expression of TRA-1-60 kind of undifferentiated marker were observed after 14 Days.
PH
DAPI/TRA-1-60
This data was obtained through the collaboration of SANPLATEC CORP. and CiRAF.
Closed / Perfusion Cell Culture System
Micro Pump Kit
Accessories
In Development
